A new endophytic insect-associated Daldinia species, recognised from a comparison of secondary metabolite profiles and molecular phylogeny
Xylariaceous endophytes are commonly encountered in plants, and it has recently been found out that insect vectors play a major role in their life cycle, as well as in the apparent host-specificity of the fungal stromata. In this study, a new insect-associated, endophytic anamorph species of Daldini...
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Veröffentlicht in: | Fungal diversity 2013-05, Vol.60 (1), p.107-123 |
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Sprache: | eng |
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Zusammenfassung: | Xylariaceous endophytes are commonly encountered in plants, and it has recently been found out that insect vectors play a major role in their life cycle, as well as in the apparent host-specificity of the fungal stromata. In this study, a new insect-associated, endophytic anamorph species of
Daldinia
is recognised from a comparison of
a)
morphological and ecological data;
b)
molecular phylogenetic data based on rDNA, alpha-actin and beta-tubulin genes; and
c)
secondary metabolite profiles based on high performance liquid chromatography/diode array/mass spectrometry (HPLC-DAD/MS) and gas chromatography/mass spectrometry (GC-MS) in comparison with authentic strains of
Daldinia
and other Xylariaceae. The new species,
Daldinia hawksworthii,
is a symbiont of the willow woodwasp,
Xiphydria prolongata
, which is in turn preferentially associated with
Salix
. A comparison of HPLC-DAD/MS profiles from standardised cultures revealed the presence of a specific compound, which was not encountered in any of the ca. 250
Daldinia
strains studied for comparison. This compound was isolated after fermentation of the fungus in 10 l scale by preparative chromatography. Structure elucidation by NMR spectroscopy revealed a novel pyrone, for which the name dalsymbiopyrone is proposed. The compound showed weak antimicrobial and cytotoxic effects and might be a signal molecule involved in the trilateral ecological interaction between the host plant, the insect, and its fungal symbiont. |
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ISSN: | 1560-2745 1878-9129 |
DOI: | 10.1007/s13225-013-0238-5 |