Transgenic tobacco plants expressing endo-β-mannanase gene from deep-sea Bacillus sp. JAMB-602 strain confer enhanced resistance against fungal pathogen (Fusarium oxysporum)
Genes encoding pathogenesis-related proteins, such as degrading enzymes of fungal cell wall polysaccharides, have been used to confer enhanced resistance to fungal pathogens of various plants. A new type of endo-β-mannanase gene, amn5A , was isolated from alkaliphilic Bacillus strain (JAMB-602) foun...
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Veröffentlicht in: | Plant biotechnology reports 2012-07, Vol.6 (3), p.243-250 |
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Sprache: | eng |
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Zusammenfassung: | Genes encoding pathogenesis-related proteins, such as degrading enzymes of fungal cell wall polysaccharides, have been used to confer enhanced resistance to fungal pathogens of various plants. A new type of endo-β-mannanase gene,
amn5A
, was isolated from alkaliphilic
Bacillus
strain (JAMB-602) found in deep-sea sediment. The AMN5A mannanase is active over a wide pH range (pH 7–10) and stable at high temperature. In this study, transgenic tobacco plants expressing the
amn5A
gene were generated using
Agrobacterium
-mediated transformation. Polymerase chain reaction (PCR) analysis revealed that the
amn5A
gene was integrated into the genome of transgenic tobacco plants. Southern blot analysis showed that transgenic plants contained 1–6 copies of
amn5A
transgenes in their genome. Expression of the
amn5A
transgene was confirmed by reverse transcription-PCR analysis. Leaf extracts from the transgenic plants showed degradation activity of Konjak mannan. Antifungal assay of detached leaves and in vitro whole plantlets indicated that transgenic plants expressing
amn5A
gene acquired enhanced resistance to the soil-borne pathogenic fungus,
Fusarium oxysporum
, compared to untransformed control plants. |
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ISSN: | 1863-5466 1863-5474 |
DOI: | 10.1007/s11816-012-0219-2 |