Identification of biomarkers for Mycobacterium tuberculosis infection and disease in BCG-vaccinated young children in Southern India

Pediatric tuberculosis (TB) often goes undiagnosed because of the lack of reliable diagnostic methods. With the aim of assessing biomarker(s) that can aid in the diagnosis of TB infection and disease, we investigated 746 Indian children with suspected TB. Whole-blood mRNA from 210 children was exami...

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Veröffentlicht in:	Genes and immunity 2013-09, Vol.14 (6), p.356-364
Hauptverfasser:	Dhanasekaran, S, Jenum, S, Stavrum, R, Ritz, C, Faurholt-Jepsen, D, Kenneth, J, Vaz, M, Grewal, H M S, Doherty, T M
Format:	Artikel
Sprache:	eng
Schlagworte:	631/250/255/1856 692/53/2421 692/700/1720 BCG Vaccine - therapeutic use Biological markers Biomarkers Biomarkers - metabolism Biomedical and Life Sciences Biomedicine Cancer Research Carrier Proteins - genetics Carrier Proteins - metabolism Case-Control Studies CCR7 protein CD4 antigen CD4 Antigens - genetics CD4 Antigens - metabolism Chemokine CXCL10 - genetics Chemokine CXCL10 - metabolism Chemokines Child, Preschool Children Cohort analysis CXCL10 protein Cytokines Dentistry Diagnosis Disease Diseases Female Forkhead Transcription Factors - genetics Forkhead Transcription Factors - metabolism Foxp3 protein Gene Expression Genes Genetic aspects Human Genetics Humans Identification and classification Immunology India Infant Infant, Newborn Infections Interleukin 13 Interleukin 2 Interleukin-13 - genetics Interleukin-13 - metabolism Interleukin-2 - genetics Interleukin-2 - metabolism Male Mycobacterial infections Mycobacterium tuberculosis original-article Pediatrics rab GTP-Binding Proteins - genetics rab GTP-Binding Proteins - metabolism Receptors, Tumor Necrosis Factor, Type I - genetics Receptors, Tumor Necrosis Factor, Type I - metabolism RNA, Messenger - genetics RNA, Messenger - metabolism Standard scores Transcription Transforming Growth Factor beta1 - genetics Transforming Growth Factor beta1 - metabolism Tuberculosis Tuberculosis - diagnosis Tuberculosis - metabolism Tuberculosis - prevention & control
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Zusammenfassung:	Pediatric tuberculosis (TB) often goes undiagnosed because of the lack of reliable diagnostic methods. With the aim of assessing biomarker(s) that can aid in the diagnosis of TB infection and disease, we investigated 746 Indian children with suspected TB. Whole-blood mRNA from 210 children was examined by dual-color Reverse-Transcriptase Multiple Ligation-dependent Probe-Amplification for the expression of 45 genes and a Bio-Plex assay for the expression of cytokines/chemokines in QuantiFERON supernatants. The study shows that transcription of SEC14L1, GUSB, BPI, CCR7 and TGFβ-1 (all P ⩽0.05) was downregulated in TB disease compared with uninfected controls, while transcription of RAB33A was downregulated in TB disease compared with both latent TB ( P
ISSN:	1466-4879 1476-5470
DOI:	10.1038/gene.2013.26