Enhanced stability of a 2μ-based recombinant plasmid in diploid yeast
The stability of a 2 mu -based recombinant plasmid, pJDB219, has been compared in glucose-limited chemostat cultures of two haploid strains of Saccharomyces cerevisiae and a diploid derived from them. The stability of the recombinant plasmid differed in the two haploid hosts but was greatest in the...
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| Veröffentlicht in: | Biotechnology letters 1986-06, Vol.8 (6), p.391-396 |
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| container_end_page | 396 |
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| container_issue | 6 |
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| container_title | Biotechnology letters |
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| creator | MEAD, D. J GARDNER, D. C. J OLIVER, S. G |
| description | The stability of a 2 mu -based recombinant plasmid, pJDB219, has been compared in glucose-limited chemostat cultures of two haploid strains of Saccharomyces cerevisiae and a diploid derived from them. The stability of the recombinant plasmid differed in the two haploid hosts but was greatest in the diploid. Enhanced stability in the diploid is probably a function of both the increased copy number and reduced selective burden of the plasmid. |
| doi_str_mv | 10.1007/BF01026738 |
| format | Article |
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G</creatorcontrib><title>Enhanced stability of a 2μ-based recombinant plasmid in diploid yeast</title><title>Biotechnology letters</title><description>The stability of a 2 mu -based recombinant plasmid, pJDB219, has been compared in glucose-limited chemostat cultures of two haploid strains of Saccharomyces cerevisiae and a diploid derived from them. The stability of the recombinant plasmid differed in the two haploid hosts but was greatest in the diploid. Enhanced stability in the diploid is probably a function of both the increased copy number and reduced selective burden of the plasmid.</description><subject>Biological and medical sciences</subject><subject>Biotechnology</subject><subject>diploids</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Genetic engineering</subject><subject>Genetic technics</subject><subject>Methods. Procedures. 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| fulltext | fulltext |
| identifier | ISSN: 0141-5492 |
| ispartof | Biotechnology letters, 1986-06, Vol.8 (6), p.391-396 |
| issn | 0141-5492 1573-6776 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_14389729 |
| source | SpringerNature Journals |
| subjects | Biological and medical sciences Biotechnology diploids Fundamental and applied biological sciences. Psychology Genetic engineering Genetic technics Methods. Procedures. Technologies Miscellaneous plasmids Saccharomyces cerevisiae Synthetic digonucleotides and genes. Sequencing |
| title | Enhanced stability of a 2μ-based recombinant plasmid in diploid yeast |
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