Enhanced stability of a 2μ-based recombinant plasmid in diploid yeast

The stability of a 2 mu -based recombinant plasmid, pJDB219, has been compared in glucose-limited chemostat cultures of two haploid strains of Saccharomyces cerevisiae and a diploid derived from them. The stability of the recombinant plasmid differed in the two haploid hosts but was greatest in the...

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Veröffentlicht in:	Biotechnology letters 1986-06, Vol.8 (6), p.391-396
Hauptverfasser:	MEAD, D. J, GARDNER, D. C. J, OLIVER, S. G
Format:	Artikel
Sprache:	eng
Schlagworte:	Biological and medical sciences Biotechnology diploids Fundamental and applied biological sciences. Psychology Genetic engineering Genetic technics Methods. Procedures. Technologies Miscellaneous plasmids Saccharomyces cerevisiae Synthetic digonucleotides and genes. Sequencing
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Zusammenfassung:	The stability of a 2 mu -based recombinant plasmid, pJDB219, has been compared in glucose-limited chemostat cultures of two haploid strains of Saccharomyces cerevisiae and a diploid derived from them. The stability of the recombinant plasmid differed in the two haploid hosts but was greatest in the diploid. Enhanced stability in the diploid is probably a function of both the increased copy number and reduced selective burden of the plasmid.
ISSN:	0141-5492 1573-6776
DOI:	10.1007/BF01026738