Cloning and characterization of respiratory syncytial virus cDNA.
Genomic RNA of the Respiratory syntical virus (RS-virus) was used as a template to synthesize calfthymus DNA-primed cDNAs. These cDNAs were subsequently cloned into the E. coli plasmid pBR 322 at the PstI-site. By hybridization 6 recombinants with virus-specific cDNAs representing 10% of the whole g...
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| Veröffentlicht in: | Acta biotechnologica 1986-01, Vol.6 (1), p.10-13 |
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| container_title | Acta biotechnologica |
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| creator | Proesch, S Seidel, W Doehner, L Liebscher, D H |
| description | Genomic RNA of the Respiratory syntical virus (RS-virus) was used as a template to synthesize calfthymus DNA-primed cDNAs. These cDNAs were subsequently cloned into the E. coli plasmid pBR 322 at the PstI-site. By hybridization 6 recombinants with virus-specific cDNAs representing 10% of the whole genome could be isolated. |
| format | Article |
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These cDNAs were subsequently cloned into the E. coli plasmid pBR 322 at the PstI-site. 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These cDNAs were subsequently cloned into the E. coli plasmid pBR 322 at the PstI-site. By hybridization 6 recombinants with virus-specific cDNAs representing 10% of the whole genome could be isolated.</description><subject>cDNA</subject><subject>cloning</subject><subject>Escherichia coli</subject><subject>respiratory syncytial virus</subject><issn>0138-4988</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1986</creationdate><recordtype>article</recordtype><recordid>eNqNyr0OgjAQAOAOmog_79DJDdMCJnUkqHFycieXWvRMbfGumODTu_gATt_yTUSmdGnyamfMTMyZH0pprXSRibrxMWC4SQhXae9AYJMj_EDCGGTsJDnukSBFGiWPwY4Jwcs30sDS7s_1ZimmHXh2q58LsT4eLs0p7ym-BsepfSJb5z0EFwdudVWaymyL8u_4BczGPGI</recordid><startdate>19860101</startdate><enddate>19860101</enddate><creator>Proesch, S</creator><creator>Seidel, W</creator><creator>Doehner, L</creator><creator>Liebscher, D H</creator><scope>7QO</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope></search><sort><creationdate>19860101</creationdate><title>Cloning and characterization of respiratory syncytial virus cDNA.</title><author>Proesch, S ; Seidel, W ; Doehner, L ; Liebscher, D H</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-proquest_miscellaneous_143848523</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1986</creationdate><topic>cDNA</topic><topic>cloning</topic><topic>Escherichia coli</topic><topic>respiratory syncytial virus</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Proesch, S</creatorcontrib><creatorcontrib>Seidel, W</creatorcontrib><creatorcontrib>Doehner, L</creatorcontrib><creatorcontrib>Liebscher, D H</creatorcontrib><collection>Biotechnology Research Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><jtitle>Acta biotechnologica</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Proesch, S</au><au>Seidel, W</au><au>Doehner, L</au><au>Liebscher, D H</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Cloning and characterization of respiratory syncytial virus cDNA.</atitle><jtitle>Acta biotechnologica</jtitle><date>1986-01-01</date><risdate>1986</risdate><volume>6</volume><issue>1</issue><spage>10</spage><epage>13</epage><pages>10-13</pages><issn>0138-4988</issn><abstract>Genomic RNA of the Respiratory syntical virus (RS-virus) was used as a template to synthesize calfthymus DNA-primed cDNAs. These cDNAs were subsequently cloned into the E. coli plasmid pBR 322 at the PstI-site. By hybridization 6 recombinants with virus-specific cDNAs representing 10% of the whole genome could be isolated.</abstract></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0138-4988 |
| ispartof | Acta biotechnologica, 1986-01, Vol.6 (1), p.10-13 |
| issn | 0138-4988 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_14384852 |
| source | Wiley Online Library All Journals |
| subjects | cDNA cloning Escherichia coli respiratory syncytial virus |
| title | Cloning and characterization of respiratory syncytial virus cDNA. |
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