Agrobacterium tumefaciens-mediated genetic transformation of the Taxol-producing endophytic fungus Ozonium sp EFY21

An efficient Agrobacterium tumefaciens-mediated genetic transformation method was successfully established for a newly isolated Taxol-producing fungus, Ozonium sp EFY21. A specific hygromycin B resistance expression vector, pCAMBIA1304'AN7-1, was constructed for fungal transformation. Key facto...

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Veröffentlicht in:	Genetics and molecular research 2013-08, Vol.12 (3), p.2913-2922
Hauptverfasser:	Liu, L, Wei, Y M, Zhou, X W, Lin, J, Sun, X F, Tang, K X
Format:	Artikel
Sprache:	eng
Schlagworte:	Agrobacterium tumefaciens - genetics Agrobacterium tumefaciens - metabolism Ascomycota - genetics Ascomycota - metabolism Endophytes - genetics Endophytes - metabolism Paclitaxel - biosynthesis Protoplasts - metabolism Transformation, Genetic
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description	An efficient Agrobacterium tumefaciens-mediated genetic transformation method was successfully established for a newly isolated Taxol-producing fungus, Ozonium sp EFY21. A specific hygromycin B resistance expression vector, pCAMBIA1304'AN7-1, was constructed for fungal transformation. Key factors affecting transformation efficiency were thoroughly investigated and optimized. PCR amplification and Southern hybridization were used to verify the transformation events. This study should pave the way for future genetic modification studies of Ozonium sp EFY21.
doi_str_mv	10.4238/2013.August.12.7
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This study should pave the way for future genetic modification studies of Ozonium sp EFY21.</description><subject>Agrobacterium tumefaciens - genetics</subject><subject>Agrobacterium tumefaciens - metabolism</subject><subject>Ascomycota - genetics</subject><subject>Ascomycota - metabolism</subject><subject>Endophytes - genetics</subject><subject>Endophytes - metabolism</subject><subject>Paclitaxel - biosynthesis</subject><subject>Protoplasts - metabolism</subject><subject>Transformation, Genetic</subject><issn>1676-5680</issn><issn>1676-5680</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2013</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpNkDtPwzAUhS0E4r0zIY8sKX7FSceqooBUqQsMTJZjX5egxi5-SMCvJ1UBMd07nO_o6EPoipKJYLy9ZYTyyaysS8oTyibNATqlspFVLVty-O8_QWcpvRHCatGSY3TCBJG1FM0pSrN1DJ02GWJfBpzLAE6bHnyqBrC9zmDxGjzk3uActU8uxEHnPngcHM6vgJ_0R9hU2xhsMb1fY_A2bF8_d4ArfpyGV1_B78rTFt8tXhi9QEdObxJc_txz9Ly4e5o_VMvV_eN8tqwMb9tcWdCGE94xYkAKTk3NplPHu6k0RFpqjQBHnBStbYWh1DaE2GlnWatr14Ex_Bzd7HvHce8FUlZDnwxsNtpDKElRwRtKG0LZGCX7qIkhpQhObWM_6PipKFE71WqnWu1VK8pUMyLXP-2lG1X9Ab9u-Tdnf35i</recordid><startdate>20130812</startdate><enddate>20130812</enddate><creator>Liu, L</creator><creator>Wei, Y M</creator><creator>Zhou, X W</creator><creator>Lin, J</creator><creator>Sun, X F</creator><creator>Tang, K X</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20130812</creationdate><title>Agrobacterium tumefaciens-mediated genetic transformation of the Taxol-producing endophytic fungus Ozonium sp EFY21</title><author>Liu, L ; Wei, Y M ; Zhou, X W ; Lin, J ; Sun, X F ; Tang, K X</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c388t-deac303b20ce6431c5299f3b96c06d1dc4ef0f648d84c11d700d9bd28a5fbecc3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2013</creationdate><topic>Agrobacterium tumefaciens - genetics</topic><topic>Agrobacterium tumefaciens - metabolism</topic><topic>Ascomycota - genetics</topic><topic>Ascomycota - metabolism</topic><topic>Endophytes - genetics</topic><topic>Endophytes - metabolism</topic><topic>Paclitaxel - biosynthesis</topic><topic>Protoplasts - metabolism</topic><topic>Transformation, Genetic</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Liu, L</creatorcontrib><creatorcontrib>Wei, Y M</creatorcontrib><creatorcontrib>Zhou, X W</creatorcontrib><creatorcontrib>Lin, J</creatorcontrib><creatorcontrib>Sun, X F</creatorcontrib><creatorcontrib>Tang, K X</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Genetics and molecular research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Liu, L</au><au>Wei, Y M</au><au>Zhou, X W</au><au>Lin, J</au><au>Sun, X F</au><au>Tang, K X</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Agrobacterium tumefaciens-mediated genetic transformation of the Taxol-producing endophytic fungus Ozonium sp EFY21</atitle><jtitle>Genetics and molecular research</jtitle><addtitle>Genet Mol Res</addtitle><date>2013-08-12</date><risdate>2013</risdate><volume>12</volume><issue>3</issue><spage>2913</spage><epage>2922</epage><pages>2913-2922</pages><issn>1676-5680</issn><eissn>1676-5680</eissn><abstract>An efficient Agrobacterium tumefaciens-mediated genetic transformation method was successfully established for a newly isolated Taxol-producing fungus, Ozonium sp EFY21. 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subjects	Agrobacterium tumefaciens - genetics Agrobacterium tumefaciens - metabolism Ascomycota - genetics Ascomycota - metabolism Endophytes - genetics Endophytes - metabolism Paclitaxel - biosynthesis Protoplasts - metabolism Transformation, Genetic
title	Agrobacterium tumefaciens-mediated genetic transformation of the Taxol-producing endophytic fungus Ozonium sp EFY21
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