Agrobacterium tumefaciens-mediated genetic transformation of the Taxol-producing endophytic fungus Ozonium sp EFY21

Agrobacterium tumefaciens-mediated genetic transformation of the Taxol-producing endophytic fungus Ozonium sp EFY21

An efficient Agrobacterium tumefaciens-mediated genetic transformation method was successfully established for a newly isolated Taxol-producing fungus, Ozonium sp EFY21. A specific hygromycin B resistance expression vector, pCAMBIA1304'AN7-1, was constructed for fungal transformation. Key facto...

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Veröffentlicht in:Genetics and molecular research 2013-08, Vol.12 (3), p.2913-2922
Hauptverfasser: Liu, L, Wei, Y M, Zhou, X W, Lin, J, Sun, X F, Tang, K X
Format: Artikel
Sprache:eng
Schlagworte:
Agrobacterium tumefaciens - genetics
Agrobacterium tumefaciens - metabolism
Ascomycota - genetics
Ascomycota - metabolism
Endophytes - genetics
Endophytes - metabolism
Paclitaxel - biosynthesis
Protoplasts - metabolism
Transformation, Genetic
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Zusammenfassung:An efficient Agrobacterium tumefaciens-mediated genetic transformation method was successfully established for a newly isolated Taxol-producing fungus, Ozonium sp EFY21. A specific hygromycin B resistance expression vector, pCAMBIA1304'AN7-1, was constructed for fungal transformation. Key factors affecting transformation efficiency were thoroughly investigated and optimized. PCR amplification and Southern hybridization were used to verify the transformation events. This study should pave the way for future genetic modification studies of Ozonium sp EFY21.
ISSN:1676-5680
1676-5680
DOI:10.4238/2013.August.12.7