Broad host range cloning vectors for Gram-negative bacteria

A series of cloning vectors has been constructed based on the broad-host-range plasmid R300B. One of these vectors, pGSS33, has a size of 13.4kb and carries four antibiotic resistance genes [ampicillin (Ap r), chloramphenicol (Cm r), streptomycin (Sm r) and tetracycline (Tc r) L all of which have re...

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Veröffentlicht in:	Gene 1984-01, Vol.29 (1), p.93-102
1. Verfasser:	Sharpe, Geoffrey S.
Format:	Artikel
Sprache:	eng
Schlagworte:	Anti-Bacterial Agents - toxicity antibiotic resistance Base Sequence Biological and medical sciences Biotechnology cloning cloning vectors Cloning, Molecular DNA Restriction Enzymes Drug Resistance, Microbial Escherichia coli Escherichia coli - genetics Euryarchaeota - genetics Fundamental and applied biological sciences. Psychology genes Genes, Bacterial Genetic engineering Genetic technics Genetic Vectors gram-negative bacteria Gram-Negative Bacteria - genetics insertional inactivation Methods. Procedures. Technologies multiple cloning sites plasmids R Factors R300B Recombinant DNA restriction analysis Vectors (cloning, transfer, expression). Insertion sequences and transposons
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Beschreibung
Zusammenfassung:	A series of cloning vectors has been constructed based on the broad-host-range plasmid R300B. One of these vectors, pGSS33, has a size of 13.4kb and carries four antibiotic resistance genes [ampicillin (Ap r), chloramphenicol (Cm r), streptomycin (Sm r) and tetracycline (Tc r) L all of which have restriction sites for insertional inactivation. The derivation, structure and uses of the plasmids are described.
ISSN:	0378-1119 1879-0038
DOI:	10.1016/0378-1119(84)90170-7