The Biological Activity of H. Pylori SlyD in Vitro

Aim To investigate the biological activity of the H. pylori SlyD in vitro. Methods Helicobacter pylori (H.pylori) slyD prokaryotic expression vector was carried out in Escherichia coli (E.coli), and recombination SlyD (rSlyD) was purified by immobilized metal affinity chromatography. The proliferati...

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Veröffentlicht in:	Helicobacter (Cambridge, Mass.) Mass.), 2013-10, Vol.18 (5), p.347-355
Hauptverfasser:	Dan, Kang, Yuehua, Gong, yanmei, Zhu, aodi, Li, nannan, Dong, ying, Piao, Yuan, Yuan
Format:	Artikel
Sprache:	eng
Schlagworte:	Apoptosis - drug effects Caspase 8 - metabolism Cell Line, Tumor Cell Proliferation - drug effects Cell Transformation, Neoplastic - drug effects Epithelial Cells - drug effects Escherichia coli Escherichia coli - genetics FK506-binding protein gastric cancer Gene Expression Gene Expression Profiling Helicobacter pylori Helicobacter pylori - enzymology Humans peptidyl-prolyl isomerase Peptidylprolyl Isomerase - genetics Peptidylprolyl Isomerase - isolation & purification Peptidylprolyl Isomerase - metabolism Recombinant Proteins - genetics Recombinant Proteins - isolation & purification Recombinant Proteins - metabolism Sincalide - metabolism slyD Virulence Factors - genetics Virulence Factors - isolation & purification Virulence Factors - metabolism
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Zusammenfassung:	Aim To investigate the biological activity of the H. pylori SlyD in vitro. Methods Helicobacter pylori (H.pylori) slyD prokaryotic expression vector was carried out in Escherichia coli (E.coli), and recombination SlyD (rSlyD) was purified by immobilized metal affinity chromatography. The proliferation, apoptosis, invasion, transformation effects of rSlyD on AGS cells was detected by CCK‐8, cell cycle, caspase‐3 activity, matrigel invasion assay, and double‐deck soft agar colony forming efficiency. In addition, the expressions of PCNA, KI‐67, caspase‐3, and MMP‐9 were detected by western blot and immunofluorescence assay, respectively. Results The CCK‐8 assay revealed that cell proliferation was increased in a time and dose‐dependent manner in AGS + rSlyD group compared with that of AGS or AGS + PBS group (p
ISSN:	1083-4389 1523-5378
DOI:	10.1111/hel.12057