Enzyme immunoassay microtiter plate response to atrazine and metolachlor in potentially interfering matrices

Standard enzyme immunoassay analysis atrazine and metolachlor responses were studied at various dilutions of organic solvents and with humic acids. Solutions containing less than 3.5% acetonitrile had no adverse effect on atrazine standard curves based on similarity of slopes of standard lines. No difference in slope was observed for atrazine standards in 0.4 3.5% methanol solutions. At 0.2 ng/ml atrazine, humic acids interfered with the enzyme immunoassay response.