A new enzyme-linked immunosorbent assay system for human hepatic triglyceride lipase

The objective of this study was to establish a new sandwich based enzyme linked immunosorbent assay (ELISA) for measuring the protein mass of human hepatic triacylglyceride lipase (HTGL). Two mouse monoclonal antibodies raised against human HTGL were used for the sandwich ELISA. The post-heparin pla...

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Veröffentlicht in:	Clinica chimica acta 2013-09, Vol.424, p.201-206
Hauptverfasser:	Miyashita, Kazuya, Kobayashi, Junji, Imamura, Shigeyuki, Kinoshita, Noriaki, Stanhope, Kimber L, Havel, Peter J, Nakajima, Katsuyuki, Machida, Tetsuo, Sumino, Hiroyuki, Nara, Makoto, Murakami, Masami
Format:	Artikel
Sprache:	eng
Schlagworte:	Adult Animals Antibodies, Monoclonal - biosynthesis Antibodies, Monoclonal - chemistry Antibodies, Monoclonal - isolation & purification CHO Cells Cholesterol, HDL - blood Cholesterol, LDL - blood Cricetulus ELISA Endothelial lipase Enzyme-Linked Immunosorbent Assay Female Hepatic triglyceride lipase Humans Lipase - blood Liver - enzymology Male Mice Obesity - blood Recombinant Proteins - analysis Reproducibility of Results Sensitivity and Specificity Triglycerides - blood
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Zusammenfassung:	The objective of this study was to establish a new sandwich based enzyme linked immunosorbent assay (ELISA) for measuring the protein mass of human hepatic triacylglyceride lipase (HTGL). Two mouse monoclonal antibodies raised against human HTGL were used for the sandwich ELISA. The post-heparin plasma (PHP) samples obtained at a heparin dose of 50unit/kg from 124 normolipidemic subjects were used for this ELISA. The dynamic assay range of the developed ELISA for the HTGL was from 0.47 to 30ng/ml. The CV was
ISSN:	0009-8981 1873-3492
DOI:	10.1016/j.cca.2013.06.016