Translational repression using BIV Tat peptide-TAR RNA interaction in mammalian cells

Translational repression using BIV Tat peptide-TAR RNA interaction in mammalian cells

We developed a strategy to create novel genetically encoded switches based on translational repression. We illustrated its efficacy by incorporating two copies of an RNA hairpin in the 5'-untranslated region (UTR) of a target mRNA and demonstrating 7-fold translational repression upon expressio...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Veröffentlicht in:Chemical communications (Cambridge, England) England), 2013-01, Vol.49 (67), p.7457-7459
Hauptverfasser: Sudrik, Chaitanya, Arha, Manish, Cao, Jicong, Schaffer, David V, Kane, Ravi S
Format: Artikel
Sprache:eng
Schlagworte:
5' Untranslated Regions
Animals
Cell Line
Gene Expression Regulation
Immunodeficiency Virus, Bovine - genetics
Peptides - genetics
Protein Biosynthesis
RNA, Messenger - chemistry
RNA, Messenger - genetics
RNA, Viral - genetics
Viral Proteins - genetics
Online-Zugang:Volltext
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
Beschreibung
Zusammenfassung:We developed a strategy to create novel genetically encoded switches based on translational repression. We illustrated its efficacy by incorporating two copies of an RNA hairpin in the 5'-untranslated region (UTR) of a target mRNA and demonstrating 7-fold translational repression upon expression of a ligand - the BIV Tat peptide.
ISSN:1359-7345
1364-548X
DOI:10.1039/c3cc43086c