Explant establishment for callus initiation of a Nigerian endangered leafy vegetable, Gnetum africanum (WILLD)

A prerequisite for successful in vitro culture is the establishment of an aseptic technique, thus the experiment was to investigate suitable sterilization regimes for the leaf explants of Gnetum africanum, an endangered green leafy vegetable. Three sterilization regimes were tested to establish the best regime using three to four days old leaves. The surface sterilized explants were later aseptically introduced onto the surfaces of sterile Murashige and Skoog agar media, incubated at 25 degree C for three weeks in the growth chamber. 100% sterility was observed from the regime which was significantly different (P