Preservation and Conidia Culture Technique of Ustilaginoidea virens
[Objective] This study aimed to find methods suitable for the preservation and conidia production of Ustilaginoidea virens. [Method] Six different preservation methods were tested by checking at three month interval for the viability and sporulation, and seven liquid media of different composition w...
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| Veröffentlicht in: | Hunan agricultural science & technology newsletter : HASTN 2013-04, Vol.14 (4), p.624-624 |
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| container_title | Hunan agricultural science & technology newsletter : HASTN |
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| creator | He, Haiyong Wongkaew, Sopone Buensanteai, Natthiya Yang, Xuehui Chen, Xiaojun Wu, Shiping Tan, Qingqun Wang, Lishuang Yuan, Jie |
| description | [Objective] This study aimed to find methods suitable for the preservation and conidia production of Ustilaginoidea virens. [Method] Six different preservation methods were tested by checking at three month interval for the viability and sporulation, and seven liquid media of different composition were tested by shaking culture at 150 r/min. [Result] Among the six methods studied, the periodic transfer and paraffin oil overlay were suitable, and the fungus could survive more than 12 months. The conidia concentration went up to 7.25×10^sup 7^ conidia/ml after 9 d in the PSB liquid medium, indicating that PSB was the best medium for conidia production. [Conclusion] This study provides a theoretical basis for the preservation and conidia production of U. virens. [PUBLICATION ABSTRACT] |
| format | Article |
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[Method] Six different preservation methods were tested by checking at three month interval for the viability and sporulation, and seven liquid media of different composition were tested by shaking culture at 150 r/min. [Result] Among the six methods studied, the periodic transfer and paraffin oil overlay were suitable, and the fungus could survive more than 12 months. The conidia concentration went up to 7.25×10^sup 7^ conidia/ml after 9 d in the PSB liquid medium, indicating that PSB was the best medium for conidia production. [Conclusion] This study provides a theoretical basis for the preservation and conidia production of U. virens. [PUBLICATION ABSTRACT]</description><identifier>ISSN: 1009-4229</identifier><language>eng</language><publisher>Changsha: Wu Chu (USA-China) Science and Culture Media Corporation</publisher><subject>Disease ; Experiments ; Fungi ; Glycerol ; Grain ; Methods ; Sucrose ; Ustilaginoidea virens</subject><ispartof>Hunan agricultural science & technology newsletter : HASTN, 2013-04, Vol.14 (4), p.624-624</ispartof><rights>Copyright Wu Chu (USA-China) Science and Culture Media Corporation Apr 2013</rights><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780</link.rule.ids></links><search><creatorcontrib>He, Haiyong</creatorcontrib><creatorcontrib>Wongkaew, Sopone</creatorcontrib><creatorcontrib>Buensanteai, Natthiya</creatorcontrib><creatorcontrib>Yang, Xuehui</creatorcontrib><creatorcontrib>Chen, Xiaojun</creatorcontrib><creatorcontrib>Wu, Shiping</creatorcontrib><creatorcontrib>Tan, Qingqun</creatorcontrib><creatorcontrib>Wang, Lishuang</creatorcontrib><creatorcontrib>Yuan, Jie</creatorcontrib><title>Preservation and Conidia Culture Technique of Ustilaginoidea virens</title><title>Hunan agricultural science & technology newsletter : HASTN</title><description>[Objective] This study aimed to find methods suitable for the preservation and conidia production of Ustilaginoidea virens. [Method] Six different preservation methods were tested by checking at three month interval for the viability and sporulation, and seven liquid media of different composition were tested by shaking culture at 150 r/min. [Result] Among the six methods studied, the periodic transfer and paraffin oil overlay were suitable, and the fungus could survive more than 12 months. The conidia concentration went up to 7.25×10^sup 7^ conidia/ml after 9 d in the PSB liquid medium, indicating that PSB was the best medium for conidia production. [Conclusion] This study provides a theoretical basis for the preservation and conidia production of U. virens. 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| identifier | ISSN: 1009-4229 |
| ispartof | Hunan agricultural science & technology newsletter : HASTN, 2013-04, Vol.14 (4), p.624-624 |
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| language | eng |
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| source | Alma/SFX Local Collection |
| subjects | Disease Experiments Fungi Glycerol Grain Methods Sucrose Ustilaginoidea virens |
| title | Preservation and Conidia Culture Technique of Ustilaginoidea virens |
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