Preservation and Conidia Culture Technique of Ustilaginoidea virens

[Objective] This study aimed to find methods suitable for the preservation and conidia production of Ustilaginoidea virens. [Method] Six different preservation methods were tested by checking at three month interval for the viability and sporulation, and seven liquid media of different composition were tested by shaking culture at 150 r/min. [Result] Among the six methods studied, the periodic transfer and paraffin oil overlay were suitable, and the fungus could survive more than 12 months. The conidia concentration went up to 7.25×10^sup 7^ conidia/ml after 9 d in the PSB liquid medium, indicating that PSB was the best medium for conidia production. [Conclusion] This study provides a theoretical basis for the preservation and conidia production of U. virens. [PUBLICATION ABSTRACT]