NMR chemical shift assignments for androcam, a testis-specific myosin VI light chain in D. melanogaster

Androcam is a D. melanogaster calmodulin-like protein expressed exclusively in the testis that interacts with myosin VI and is critical to spermatogenesis. At micromolar free Ca 2+ , androcam binds two calcium ions using its C-terminal lobe but its N-terminal lobe is Ca 2+ -free. We are pursuing str...

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Veröffentlicht in:	Biomolecular NMR assignments 2013-10, Vol.7 (2), p.167-169
Hauptverfasser:	Joshi, Mehul K., Moran, Sean, MacKenzie, Kevin R.
Format:	Artikel
Sprache:	eng
Schlagworte:	Amino Acid Sequence Animals Biochemistry Biological and Medical Physics Biophysics Calcium Calcium-Binding Proteins - chemistry Drosophila melanogaster - metabolism Drosophila Proteins - chemistry Male Myosin Heavy Chains - chemistry NMR Nuclear magnetic resonance Nuclear Magnetic Resonance, Biomolecular Organ Specificity Physics Physics and Astronomy Polymer Sciences Proteins Spermatogenesis Testis - metabolism
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Zusammenfassung:	Androcam is a D. melanogaster calmodulin-like protein expressed exclusively in the testis that interacts with myosin VI and is critical to spermatogenesis. At micromolar free Ca 2+ , androcam binds two calcium ions using its C-terminal lobe but its N-terminal lobe is Ca 2+ -free. We are pursuing structural studies on androcam at physiological (10 μM) and high (10 mM) calcium. Here we report the 1 H, 15 N, and 13 C chemical shifts of androcam at 10 μM free Ca 2+ determined using multi-dimensional NMR experiments.
ISSN:	1874-2718 1874-270X
DOI:	10.1007/s12104-012-9402-1