Allosteric effect of protons and adenosine triphosphate on hemoglobins from aquatic amphibia

The analysis of hemoglobins from two anurans, one semi-aquatic (Leptodactylus labyrinthicus ) and the other aquatic (Pipa carvalhoi ), showed several components isolated by CM-cellulose chromatography. The two major components (Hb II and Hb III) of L. labyrinthicus and the major components (Hb III and Hb IV) of P. carvalhoi possess functional properties as follows: i. P. carvalhoi Hb III and Hb IV and L. labyrinthicus Hb II had very small Bohr effects (-0.05) and a substantial heterotropic effect with polyphosphates. ii. L. labyrinthisus Hb III produced a normal Bohr effect of -0.17, with no influence of polyphosphates.