Age-related reduction of structural complexity in spleen hematopoietic tissue architecture in mice

The effects of aging on structural complexity in hematopoietic tissue are unknown. In this work, in a mouse experimental model, we report the age-related reduction of spleen hematopoietic tissue (SHT) complexity. Spleen tissue was obtained from the total of 64 male Swiss albino mice divided into 8 age groups: newborns (0days old), 10days, 20days, 30days, 120days, 210days, 300 and 390days old. SHT was stained using conventional hematoxylin/eosin, and DNA-binding toluidine blue dyes. Fractal dimension as an indicator of cellular complexity, and lacunarity as indicator of tissue heterogeneity were determined based on the binarized SHT micrographs. Results indicate that fractal dimension of mice spleen hematopoietic tissue decreases with age, while lacunarity increases. These changes/trends have been detected in SHT stained both with toluidine blue and conventional hematoxylin/eosin. Fractal dimension was negatively correlated with lacunarity. The detected reduction in complexity suggests that age-related structural changes are present in mouse SHT both in general tissue architecture and progenitor cell DNA. •Effects of aging on mouse spleen hematopoietic tissue (SHT) complexity are unknown.•SHT complexity in 64 mice was measured by calculating values of fractal dimension.•Age-dependent complexity reduction was found in hematoxylin/eosin stained tissue.•Similar changes were detected in toluidine blue stained tissue.