Integrated process production and extraction of the fibrinolytic protease from Bacillus sp. UFPEDA 485

Fibrinolytic proteases are enzymes that degrade fibrin; these enzymes are a promising alternative for thrombolytic therapy, and microorganisms produce them. The aim of this study was to evaluate the optimum conditions for the integrated production and purification of fibrinolytic protease from Bacil...

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Veröffentlicht in:	Applied biochemistry and biotechnology 2013-08, Vol.170 (7), p.1676-1688
Hauptverfasser:	Sales, Amanda Emmanuelle, Souza, Fabiana América Silva Dantas de, Teixeira, J. A., Porto, Tatiana Souza, Porto, Ana L. F.
Format:	Artikel
Sprache:	eng
Schlagworte:	ATPS Bacillus Bacillus - classification Bacillus - enzymology Bacteria Biochemistry Biological and medical sciences Bioreactors - microbiology Biotechnology Chemistry Chemistry and Materials Science Computer Simulation Enzymatic activity Experimental design Extractive fermentation Fermentation Fibrinolytic Agents Fibrinolytic protease Fundamental and applied biological sciences. Psychology Methods. Procedures. Technologies Microbial engineering. Fermentation and microbial culture technology Microbiology Microorganisms Models, Biological PEG/sodium sulfate Peptide Hydrolases - chemistry Peptide Hydrolases - isolation & purification Peptide Hydrolases - metabolism Polyethylene Glycols - metabolism Proteases Science & Technology Soybeans Sulfates - metabolism Systems Integration
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Zusammenfassung:	Fibrinolytic proteases are enzymes that degrade fibrin; these enzymes are a promising alternative for thrombolytic therapy, and microorganisms produce them. The aim of this study was to evaluate the optimum conditions for the integrated production and purification of fibrinolytic protease from Bacillus sp. UFPEDA 485. Extractive fermentation was carried out in a culture medium containing soybean flour and by adding polyethylene glycol (PEG) and Na2SO4 according to a 23 experimental design. In all assays, the enzyme preferentially partitioned to the bottom phase (K
ISSN:	0273-2289 0273-2289 1559-0291
DOI:	10.1007/s12010-013-0306-z