T4 RNA ligase mediated preparation of novel "chemically misacylated" tRNA super(Phe)S

T4 RNA ligase mediated preparation of novel "chemically misacylated" tRNA super(Phe)S

T4 RNA ligase was employed for the condensation of Escherichia coli tRNA super(Phe) missing cytidine-75 and adenosine-76 (tRNA super(Phe)-C sub(OH); the acceptor "oligomer") with each of several chemically acylated derivatives of pCpA (the donor "oligomer"). The resulting "c...

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Veröffentlicht in:Biochemistry (Easton) 1984-01, Vol.23 (7), p.1468-1473
Hauptverfasser: Heckler, T G, Chang, L-H, Zama, Y, Naka, T, Chorghade, MS, Hecht, S M
Format: Artikel
Sprache:eng
Schlagworte:
phage T4
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Zusammenfassung:T4 RNA ligase was employed for the condensation of Escherichia coli tRNA super(Phe) missing cytidine-75 and adenosine-76 (tRNA super(Phe)-C sub(OH); the acceptor "oligomer") with each of several chemically acylated derivatives of pCpA (the donor "oligomer"). The resulting "chemically misacylated" tRNA super(Phe)S were obtained in 20-65% yields following chromatographic workup on DEAE-cellulose and benzoylated DEAE-cellulose.
ISSN:0006-2960