Electron transport driven phosphorylation catalyzed by proteoliposomes containing hydrogenase, fumarate reductase and ATP synthase

Liposomes containing the hydrogenase complex and the fumarate reductase complex isolated from Wolinella (formerly Vibrio) succinogenes, together with vitamin K 1 catalyzed the electron transport from H 2 to fumarate. With the fumarate reductase complex present in excess the activity of electron transport was close to that of the hydrogenase complex. Liposomes containing an ATP synthase in addition to the electron transport components catalyzed the phosphorylation of ADP driven by the electron transport.