Argonaute 1 is indispensable for juvenile hormone mediated oogenesis in the migratory locust, Locusta migratoria

Juvenile hormone (JH) is the primary hormone controlling vitellogenesis and oocyte maturation in the migratory locust Locusta migratoria, an evolutionarily primitive insect species with panoistic ovaries. However, molecular mechanisms of locust oogenesis remain unclear and the role of microRNA (miRNA) in JH mediated locust vitellogenesis and oocyte maturation has not been explored. Using miRNA sequencing and quantification with small RNA libraries derived from fat bodies of JH-deprived versus JH analog-exposed female adult locusts, we have identified 83 JH up-regulated and 60 JH down-regulated miRNAs. QRT-PCR validation has confirmed that transcription of selected miRNAs responded to JH administration and correlated with changes in endogenous hemolymph JH titers. Depletion of Argonaute 1 (Ago1), a key regulator of miRNA biogenesis and function by RNAi in female adult locusts dramatically decreased the expression of vitellogenin (Vg) and severely impaired follicular epithelium development, terminal oocyte maturation and ovarian growth. Our data indicate that Ago1 and Ago1-dependent miRNAs play a crucial role in locust vitellogenesis and egg production. [Display omitted] •microRNAs are expressed in response to juvenile hormone in locust vitellogenesis.•Juvenile hormone likely affects the transcription levels of Ago1 and Dcr1.•Ago1 depletion significantly reduces Vg expression and impairs oocyte maturation.