Impact of cardiac stem cell sheet transplantation on myocardial infarction

Purpose Myocardial infarction (MI) remains a major cause of mortality because of the limited regenerative capacity of the myocardium. Transplantation of somatic tissue-derived cells into the heart has been shown to enhance the endogenous healing process, but the magnitude of its therapeutic effects...

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Veröffentlicht in:Surgery today (Tokyo, Japan) Japan), 2013-09, Vol.43 (9), p.970-976
Hauptverfasser: Alshammary, Sfoug, Fukushima, Satsuki, Miyagawa, Shigeru, Matsuda, Takenori, Nishi, Hiroyuki, Saito, Atsuhiro, Kamata, Sokichi, Asahara, Takayuki, Sawa, Yoshiki
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Sprache:eng
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Zusammenfassung:Purpose Myocardial infarction (MI) remains a major cause of mortality because of the limited regenerative capacity of the myocardium. Transplantation of somatic tissue-derived cells into the heart has been shown to enhance the endogenous healing process, but the magnitude of its therapeutic effects is dependent upon the cell-source or cell-delivery method. We investigated the therapeutic effects of C-Kit positive cardiac cell (CSC) cell-sheet transplantation therapy in a rat model of MI. Methods and results CSCs of human origin were sorted and cultured to generate scaffold-free CSC cell-sheets. One-layered or 3-layered cell-sheets were transplanted into nude rats 1 h after left coronary artery ligation. We observed a significant increase in the left ventricular ejection fraction and a significant decrease in left ventricular systolic dimension at 2 and 4 weeks in the 3-layer group, but not in the 1-layer or sham groups. Consistently, there was less accumulation of interstitial fibrosis in the 3-layer group than in the 1-layer or sham groups. Moreover, capillary density was significantly greater in the 3-layer group than in the 1-layer or sham groups. Conclusions The 3-layered cell-sheet improved cardiac function associated with angiogenic and anti-fibrotic effects. Thus, CSC is a promising cell-source to use with the cell-sheet method for the treatment of cardiac failure, as long as a sufficient number of cells are delivered.
ISSN:0941-1291
1436-2813
DOI:10.1007/s00595-013-0528-2