Resonance Raman investigation of myoglobin and hemoglobin

The authors have measured the Raman excitation profiles (REP's) of some of the key vibrational modes of deoxy Mb and deoxy Hb. Use of new theoretical techniques that are based on the optical theorem and Kramers-Kronig relations allows to directly measure the linear Franck-Condon coupling strengths of these modes. They also demonstrate how these theoretical techniques can lead to the "deconvolution" of overlapping diffuse electronic transitions by REP measurements of vibrational modes that couple independently to the transitions of interest.