GC-MS determination of metabolites in rat kidneys
To establish a method to determine the metabolites in rat kidney tissues by gas chromatography-mass spectrometry (GC-MS) combined with chemometric techniques. Metabolites were separated and identified on HP-5MS column (30 m × 0.25 μm × 0.25 mm). The initial column temperature was 100 Celsius degree...
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| Veröffentlicht in: | Zhong nan da xue xue bao. Journal of Central South University. Yi xue ban 2013-07, Vol.38 (7), p.661-669 |
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| container_title | Zhong nan da xue xue bao. Journal of Central South University. Yi xue ban |
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| creator | Liu, Shao Wang, Fangjie Mei, Wenjuan Tao, Lijian |
| description | To establish a method to determine the metabolites in rat kidney tissues by gas chromatography-mass spectrometry (GC-MS) combined with chemometric techniques.
Metabolites were separated and identified on HP-5MS column (30 m × 0.25 μm × 0.25 mm). The initial column temperature was 100 Celsius degree lasting 3 min, and then programmed at 8 Celsius degree/ min to 300 Celsius degree, maintaining at this temperature for 6 min. The internal standard was heptadecanoic acid. The grinded kidney tissue was exacted by methanol. The supernatant was dried by nitrogen. After the oximation and derivation, the supernatant was analyzed by GC-MS. The overlapped peaks were resolved into pure chromatogram and mass spectra with chemometric techniques. Qualitative analysis was performed by comparing the obtained pure mass spectra with those in NIST mass spectra database and certificated by the standards and the references. The internal method was used for semi-quantitation.
A total of 53 compounds were identified. The main constit |
| doi_str_mv | 10.3969/j.issn.1672-7347.2013.07.002 |
| format | Article |
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Metabolites were separated and identified on HP-5MS column (30 m × 0.25 μm × 0.25 mm). The initial column temperature was 100 Celsius degree lasting 3 min, and then programmed at 8 Celsius degree/ min to 300 Celsius degree, maintaining at this temperature for 6 min. The internal standard was heptadecanoic acid. The grinded kidney tissue was exacted by methanol. The supernatant was dried by nitrogen. After the oximation and derivation, the supernatant was analyzed by GC-MS. The overlapped peaks were resolved into pure chromatogram and mass spectra with chemometric techniques. Qualitative analysis was performed by comparing the obtained pure mass spectra with those in NIST mass spectra database and certificated by the standards and the references. The internal method was used for semi-quantitation.
A total of 53 compounds were identified. The main constit</description><identifier>ISSN: 1672-7347</identifier><identifier>DOI: 10.3969/j.issn.1672-7347.2013.07.002</identifier><identifier>PMID: 23908085</identifier><language>chi</language><publisher>China</publisher><subject>Amino Acids - analysis ; Animals ; Fatty Acids - analysis ; Gas Chromatography-Mass Spectrometry ; Kidney - metabolism ; Metabolome ; Metabolomics - methods ; Rats ; Urea - analysis</subject><ispartof>Zhong nan da xue xue bao. Journal of Central South University. Yi xue ban, 2013-07, Vol.38 (7), p.661-669</ispartof><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/23908085$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Liu, Shao</creatorcontrib><creatorcontrib>Wang, Fangjie</creatorcontrib><creatorcontrib>Mei, Wenjuan</creatorcontrib><creatorcontrib>Tao, Lijian</creatorcontrib><title>GC-MS determination of metabolites in rat kidneys</title><title>Zhong nan da xue xue bao. Journal of Central South University. Yi xue ban</title><addtitle>Zhong Nan Da Xue Xue Bao Yi Xue Ban</addtitle><description>To establish a method to determine the metabolites in rat kidney tissues by gas chromatography-mass spectrometry (GC-MS) combined with chemometric techniques.
Metabolites were separated and identified on HP-5MS column (30 m × 0.25 μm × 0.25 mm). The initial column temperature was 100 Celsius degree lasting 3 min, and then programmed at 8 Celsius degree/ min to 300 Celsius degree, maintaining at this temperature for 6 min. The internal standard was heptadecanoic acid. The grinded kidney tissue was exacted by methanol. The supernatant was dried by nitrogen. After the oximation and derivation, the supernatant was analyzed by GC-MS. The overlapped peaks were resolved into pure chromatogram and mass spectra with chemometric techniques. Qualitative analysis was performed by comparing the obtained pure mass spectra with those in NIST mass spectra database and certificated by the standards and the references. The internal method was used for semi-quantitation.
A total of 53 compounds were identified. The main constit</description><subject>Amino Acids - analysis</subject><subject>Animals</subject><subject>Fatty Acids - analysis</subject><subject>Gas Chromatography-Mass Spectrometry</subject><subject>Kidney - metabolism</subject><subject>Metabolome</subject><subject>Metabolomics - methods</subject><subject>Rats</subject><subject>Urea - analysis</subject><issn>1672-7347</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2013</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNo9jz1PwzAYhD2AaFX6F5AHBpaY1x-8tkcUQUEqYgDmyG4cyZAvYmfovycShVtOOj063RFyzYFJi_b2k8WUesZRi0JLpZkALhloBiDOyPo_X5FtStEDWGslGn1BVkJaMGDu1oTvyuLljdYhh6mLvctx6OnQ0C5k54c25pBo7OnkMv2KdR-O6ZKcN65NYXvyDfl4fHgvn4r96-65vN8XIxeYC69QIgIKDUHrRi6jDuB48CiUMd4JBRKV01a5ZhEXUjWojEbgAF4KuSE3v73jNHzPIeWqi-kQ2tb1YZhTxRU3XGmOdkGvTujsu1BX4xQ7Nx2rv5vyBwQWU74</recordid><startdate>201307</startdate><enddate>201307</enddate><creator>Liu, Shao</creator><creator>Wang, Fangjie</creator><creator>Mei, Wenjuan</creator><creator>Tao, Lijian</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7X8</scope></search><sort><creationdate>201307</creationdate><title>GC-MS determination of metabolites in rat kidneys</title><author>Liu, Shao ; Wang, Fangjie ; Mei, Wenjuan ; Tao, Lijian</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-p126t-b4636606270e77f3002c0a1eb62488ba240364a794affff1234f648760100b323</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>chi</language><creationdate>2013</creationdate><topic>Amino Acids - analysis</topic><topic>Animals</topic><topic>Fatty Acids - analysis</topic><topic>Gas Chromatography-Mass Spectrometry</topic><topic>Kidney - metabolism</topic><topic>Metabolome</topic><topic>Metabolomics - methods</topic><topic>Rats</topic><topic>Urea - analysis</topic><toplevel>online_resources</toplevel><creatorcontrib>Liu, Shao</creatorcontrib><creatorcontrib>Wang, Fangjie</creatorcontrib><creatorcontrib>Mei, Wenjuan</creatorcontrib><creatorcontrib>Tao, Lijian</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>MEDLINE - Academic</collection><jtitle>Zhong nan da xue xue bao. Journal of Central South University. Yi xue ban</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Liu, Shao</au><au>Wang, Fangjie</au><au>Mei, Wenjuan</au><au>Tao, Lijian</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>GC-MS determination of metabolites in rat kidneys</atitle><jtitle>Zhong nan da xue xue bao. Journal of Central South University. Yi xue ban</jtitle><addtitle>Zhong Nan Da Xue Xue Bao Yi Xue Ban</addtitle><date>2013-07</date><risdate>2013</risdate><volume>38</volume><issue>7</issue><spage>661</spage><epage>669</epage><pages>661-669</pages><issn>1672-7347</issn><abstract>To establish a method to determine the metabolites in rat kidney tissues by gas chromatography-mass spectrometry (GC-MS) combined with chemometric techniques.
Metabolites were separated and identified on HP-5MS column (30 m × 0.25 μm × 0.25 mm). The initial column temperature was 100 Celsius degree lasting 3 min, and then programmed at 8 Celsius degree/ min to 300 Celsius degree, maintaining at this temperature for 6 min. The internal standard was heptadecanoic acid. The grinded kidney tissue was exacted by methanol. The supernatant was dried by nitrogen. After the oximation and derivation, the supernatant was analyzed by GC-MS. The overlapped peaks were resolved into pure chromatogram and mass spectra with chemometric techniques. Qualitative analysis was performed by comparing the obtained pure mass spectra with those in NIST mass spectra database and certificated by the standards and the references. The internal method was used for semi-quantitation.
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| ispartof | Zhong nan da xue xue bao. Journal of Central South University. Yi xue ban, 2013-07, Vol.38 (7), p.661-669 |
| issn | 1672-7347 |
| language | chi |
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| source | MEDLINE; Alma/SFX Local Collection |
| subjects | Amino Acids - analysis Animals Fatty Acids - analysis Gas Chromatography-Mass Spectrometry Kidney - metabolism Metabolome Metabolomics - methods Rats Urea - analysis |
| title | GC-MS determination of metabolites in rat kidneys |
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