GC-MS determination of metabolites in rat kidneys

To establish a method to determine the metabolites in rat kidney tissues by gas chromatography-mass spectrometry (GC-MS) combined with chemometric techniques. Metabolites were separated and identified on HP-5MS column (30 m × 0.25 μm × 0.25 mm). The initial column temperature was 100 Celsius degree lasting 3 min, and then programmed at 8 Celsius degree/ min to 300 Celsius degree, maintaining at this temperature for 6 min. The internal standard was heptadecanoic acid. The grinded kidney tissue was exacted by methanol. The supernatant was dried by nitrogen. After the oximation and derivation, the supernatant was analyzed by GC-MS. The overlapped peaks were resolved into pure chromatogram and mass spectra with chemometric techniques. Qualitative analysis was performed by comparing the obtained pure mass spectra with those in NIST mass spectra database and certificated by the standards and the references. The internal method was used for semi-quantitation. A total of 53 compounds were identified. The main constit