Optical imaging of non-fluorescent nanodiamonds in live cells using transient absorption microscopy

Optical imaging of non-fluorescent nanodiamonds in live cells using transient absorption microscopy

We directly observe non-fluorescent nanodiamonds in living cells using transient absorption microscopy. This label-free technology provides a novel modality to study the dynamic behavior of nanodiamonds inside the cells with intrinsic three-dimensional imaging capability. We apply this method to cap...

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Veröffentlicht in:Nanoscale 2013-06, Vol.5 (11), p.4701-4705
Hauptverfasser: Chen, Tao, Lu, Feng, Streets, Aaron M, Fei, Peng, Quan, Junmin, Huang, Yanyi
Format: Artikel
Sprache:eng
Schlagworte:
Animals
Dynamic tests
HeLa Cells
Humans
Imaging
Mice
Microscopy
Microscopy, Fluorescence
Nanocomposites
Nanodiamonds - chemistry
Nanodiamonds - ultrastructure
Nanomaterials
Nanostructure
NIH 3T3 Cells
Three dimensional
Uptakes
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Beschreibung
Zusammenfassung:We directly observe non-fluorescent nanodiamonds in living cells using transient absorption microscopy. This label-free technology provides a novel modality to study the dynamic behavior of nanodiamonds inside the cells with intrinsic three-dimensional imaging capability. We apply this method to capture the cellular uptake of nanodiamonds under various conditions, confirming the endocytosis mechanism.
ISSN:2040-3364
2040-3372
DOI:10.1039/c3nr00308f