Chemical synthesis of nonadeoxyribonucleotides with modified uracil, 5'-bromouracil and 5-methylcytosine bases using the triester method.

Chemical synthesis of nonadeoxyribonucleotides with modified uracil, 5'-bromouracil and 5-methylcytosine bases using the triester method.

In order to investigate the interaction of Eco RII restriction and modification enzymes with synthetic DNA fragments three nonadeoxyribonucleotides containing the modified bases uracil, 5-bromouracil and 5-methylcytosine were synthesized according to the phospahte triester appraoch using IPS/1-methy...

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Veröffentlicht in:Tetrahedron letters 1984-01, Vol.25 (39), p.4353-4356
Hauptverfasser: Rosenthal, A, Cech, D, Veika, V P, Drezkaja, I S, Romanova, E A, Elov, A A, Metelev, V G, Gromova, E S, Shabarova, Z A
Format: Artikel
Sprache:eng
Schlagworte:
5-bromouracil
5-methylcytosine
deoxyribonucleotides
uracil
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Zusammenfassung:In order to investigate the interaction of Eco RII restriction and modification enzymes with synthetic DNA fragments three nonadeoxyribonucleotides containing the modified bases uracil, 5-bromouracil and 5-methylcytosine were synthesized according to the phospahte triester appraoch using IPS/1-methylimidazole as the condensation agent. The patterns of these modified DNA fragments obtained by Maxam/Gilbert sequence technique are presented.
ISSN:0040-4039