The Chemokine Stromal Cell–Derived Factor-1α Promotes Endothelial Progenitor Cell–Mediated Neovascularization of Human Transplanted Fat Tissue in Diabetic Immunocompromised Mice

Stromal cell-derived factor-1α is a chemokine and mediates endothelial progenitor cell-induced neovascularization. Because vascularization of a graft is crucial for its survival, the authors investigated whether stromal cell-derived factor-1α could improve fat graft survival by inducing endothelial...

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Veröffentlicht in:Plastic and reconstructive surgery (1963) 2013-08, Vol.132 (2), p.239e-250e
Hauptverfasser: Hamed, Saher, Egozi, Dana, Dawood, Hanna, Keren, Aviad, Kruchevsky, Danny, Ben-Nun, Ohad, Gilhar, Amos, Brenner, Benjamin, Ullmann, Yehuda
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Sprache:eng
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Zusammenfassung:Stromal cell-derived factor-1α is a chemokine and mediates endothelial progenitor cell-induced neovascularization. Because vascularization of a graft is crucial for its survival, the authors investigated whether stromal cell-derived factor-1α could improve fat graft survival by inducing endothelial progenitor cell-mediated neovascularization and preventing its resorption. The authors injected 1 ml of human fat tissue into the scalps of 30 diabetic and 10 nondiabetic immunocompromised mice. The fat grafts were treated with phosphate-buffered saline or stromal cell-derived factor-1α. Determination of graft phenotype included measurements of their weights and volumes, vascular endothelial growth factor (VEGF) levels, the stromal cell-derived factor-1α receptor CXCR4, VEGF receptor 2, endothelial nitric oxide synthase, serine/threonine-specific protein kinase (protein kinase B), caspase 3, and cytochrome c expression levels, and the extent of vascularization. Eighteen days after transplantation, stromal cell-derived factor-1α treatment of the grafts in the diabetic mice (1) increased plasma VEGF levels; (2) raised VEGF receptor 2, CXCR4, endothelial nitric oxide synthase, and protein kinase B expression levels; and (3) reduced caspase 3 and cytochrome c expression levels in the fat grafts. Fifteen weeks after transplantation, stromal cell-derived factor-1α treatment of the grafts prevented their resorption and increased the extent of their vascularization. Locally delivered stromal cell-derived factor-1α increases fat graft survival by stimulating neovascularization and reducing fat cell apoptosis through an endothelial progenitor cell-mediated mechanism.
ISSN:0032-1052
1529-4242
DOI:10.1097/PRS.0b013e31829587e9