Increased Efficacies in Magnetofection and Gene Delivery to Hepatocellular Carcinoma Cells with Ternary Organic-Inorganic Hybrid Nanocomposites

A magnetic personality: Magnetofection and gene delivery have been investigated in HepG2 cells using ternary organic–inorganic hybrid nanocomposites that contain deferoxamine‐coated iron oxide nanoparticles (NPs), circular plasmid DNA, and branched polyethylenimine (PEI). It was found that the cellu...

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Veröffentlicht in:	Chemistry, an Asian journal an Asian journal, 2013-08, Vol.8 (8), p.1760-1764
Hauptverfasser:	Leung, Ken Cham-Fai, Chak, Chun-Pong, Lee, Siu-Fung, Lai, Josie M. Y., Zhu, Xiao-Ming, Wang, Yi-Xiang J., Sham, Kathy W. Y., Wong, Chi-Hin, Cheng, Christopher H. K.
Format:	Artikel
Sprache:	eng
Schlagworte:	Carcinoma, Hepatocellular - diagnostic imaging Carcinoma, Hepatocellular - metabolism Carcinoma, Hepatocellular - pathology Chemistry Ferric Compounds - chemistry gene delivery Gene Transfer Techniques Hep G2 Cells Humans imaging agents Liver Neoplasms - diagnostic imaging Liver Neoplasms - metabolism Liver Neoplasms - pathology Luciferases, Renilla - genetics Luciferases, Renilla - metabolism Magnetic Resonance Imaging Magnetics magnetofection Metal Nanoparticles - chemistry nanoparticles organic-inorganic hybrid composites Plasmids - genetics Plasmids - metabolism Polyethyleneimine - chemistry Radiography
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Zusammenfassung:	A magnetic personality: Magnetofection and gene delivery have been investigated in HepG2 cells using ternary organic–inorganic hybrid nanocomposites that contain deferoxamine‐coated iron oxide nanoparticles (NPs), circular plasmid DNA, and branched polyethylenimine (PEI). It was found that the cellular uptake efficacies depend on the ratio of components in PEI/DNA/NP, as assessed by Prussian staining, magnetic resonance imaging, and luciferase expression in the presence and absence of a magnet during transfection.
ISSN:	1861-4728 1861-471X
DOI:	10.1002/asia.201300202