Purification of C-type cytochromes from a thermophilic blue-green alga, Synechococcus vulcanus [Cyanophyceae], by hydrophobic column chromatography using toyopearls

Two C-type cytochromes, c-550 and c-553, were extracted by an improved procedure from a thermophilic blue-green alga, Synechococcus vulcanus, and effectively purified by a two-step hydrophobic chromatography. The first step was performed with a Toyopearl HW-65C :ammonium sulfate-66 column and the se...

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Veröffentlicht in:	Plant and cell physiology 1984-12, Vol.25 (8), p.1575-1578
Hauptverfasser:	Shin, M, Sakihama, N. (Kobe Univ. (Japan). Faculty of Science), Koike, H, Inoue, Y
Format:	Artikel
Sprache:	eng
Schlagworte:	Analytical, structural and metabolic biochemistry Biological and medical sciences chemical extraction chromatographic techniques CHROMATOGRAPHIE CHROMATOGRAPHY CROMATOGRAFIA CYANOPHYCEAE Cytochrome c-550 Cytochrome c-553 cytochrome c550 cytochrome c553 cytochromes EPURATION Fundamental and applied biological sciences. Psychology HAEMOGLOBIN HEMOGLOBINA HEMOGLOBINE Hemoproteins Hydrophobic chromatography Metalloproteins PLASTE PLASTIDIOS PLASTIDS Proteins PURIFICACION PURIFICATION Synechococcus vulcanus Thermophilic blue-green alga Toyopearl
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Zusammenfassung:	Two C-type cytochromes, c-550 and c-553, were extracted by an improved procedure from a thermophilic blue-green alga, Synechococcus vulcanus, and effectively purified by a two-step hydrophobic chromatography. The first step was performed with a Toyopearl HW-65C :ammonium sulfate-66 column and the second with a butyl-Toyopearl 650 column. This work is the first to apply butyl-toyopearl 650 to protein purification.
ISSN:	0032-0781 1471-9053
DOI:	10.1093/oxfordjournals.pcp.a076872