Determination of genetic variation among chickpea genotypes and their F₁ crosses with RAPD markers
The intensity of genetic diversity amongst chickpea genotypes and their crosses is unknown. The current study investigated the genetic diversity of chickpea genotypes and their F₁ crosses by using random amplified polymorphic DNA (RAPD) markers. We assessed the variation among six chickpea genotypes...
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| Veröffentlicht in: | Genetics and molecular research 2013-06, Vol.12 (2), p.2127-2137 |
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| container_title | Genetics and molecular research |
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| creator | Mahmood, K Nazeer, W Naeem, M Freed, S Khan, M A Abbas, G Iqbal, M Kulachi, A R Sajid, S A Sadiq, M A Saleem, M |
| description | The intensity of genetic diversity amongst chickpea genotypes and their crosses is unknown. The current study investigated the genetic diversity of chickpea genotypes and their F₁ crosses by using random amplified polymorphic DNA (RAPD) markers. We assessed the variation among six chickpea genotypes and 15 F₁ crosses with the RAPD markers. The six parents and their 21 hybrids were carefully studied based on the presence or absence of bands. The level of polymorphism varied with different primers. Out 28 primers used, 21 amplified the genomic DNA in all the varieties, 15 generated polymorphic bands among all the varieties, and six produced similar banding patterns. |
| doi_str_mv | 10.4238/2013.April.10.1 |
| format | Article |
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The current study investigated the genetic diversity of chickpea genotypes and their F₁ crosses by using random amplified polymorphic DNA (RAPD) markers. We assessed the variation among six chickpea genotypes and 15 F₁ crosses with the RAPD markers. The six parents and their 21 hybrids were carefully studied based on the presence or absence of bands. The level of polymorphism varied with different primers. 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| source | MEDLINE; EZB-FREE-00999 freely available EZB journals |
| subjects | Cicer - genetics Crosses, Genetic DNA Primers - metabolism Genetic Markers Genetic Variation Genotype Phylogeny Polymerase Chain Reaction Random Amplified Polymorphic DNA Technique |
| title | Determination of genetic variation among chickpea genotypes and their F₁ crosses with RAPD markers |
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