Determination of genetic variation among chickpea genotypes and their F₁ crosses with RAPD markers

Determination of genetic variation among chickpea genotypes and their F₁ crosses with RAPD markers

The intensity of genetic diversity amongst chickpea genotypes and their crosses is unknown. The current study investigated the genetic diversity of chickpea genotypes and their F₁ crosses by using random amplified polymorphic DNA (RAPD) markers. We assessed the variation among six chickpea genotypes...

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Veröffentlicht in:Genetics and molecular research 2013-06, Vol.12 (2), p.2127-2137
Hauptverfasser: Mahmood, K, Nazeer, W, Naeem, M, Freed, S, Khan, M A, Abbas, G, Iqbal, M, Kulachi, A R, Sajid, S A, Sadiq, M A, Saleem, M
Format: Artikel
Sprache:eng
Schlagworte:
Cicer - genetics
Crosses, Genetic
DNA Primers - metabolism
Genetic Markers
Genetic Variation
Genotype
Phylogeny
Polymerase Chain Reaction
Random Amplified Polymorphic DNA Technique
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Zusammenfassung:The intensity of genetic diversity amongst chickpea genotypes and their crosses is unknown. The current study investigated the genetic diversity of chickpea genotypes and their F₁ crosses by using random amplified polymorphic DNA (RAPD) markers. We assessed the variation among six chickpea genotypes and 15 F₁ crosses with the RAPD markers. The six parents and their 21 hybrids were carefully studied based on the presence or absence of bands. The level of polymorphism varied with different primers. Out 28 primers used, 21 amplified the genomic DNA in all the varieties, 15 generated polymorphic bands among all the varieties, and six produced similar banding patterns.
ISSN:1676-5680
1676-5680
DOI:10.4238/2013.April.10.1