Growth and Total Carotenoid, Chlorophyll a and Chlorophyll b of Tropical Microalgae (Isochrysis sp.) in Laboratory Cultured Conditions

A study was conducted to determine the total content of carotenoids, in Isochrysis sp. under stress condition (nitrogen starvation). Isochrysis sp. was cultured in f/2 medium at optimum light intensity (1200 Lux) with constant aeration (4.5 L min super( -1)) at 8 pH. Salinity of the water was maintained between 20-24 g L super( -1) and temperature range was 20-24 degree C. The culture of Isochrysis sp. showed a short lag phase lasting about 24 h followed by exponential phase starting from day 2 until day 10. In day 4, specific growth rate (u.) for Isochrysis sp. was 1.6059 day-1, the highest rate compared to other day. Stress parameter (N0 sub( 3) super( -) starvation) was introduced into the mass culture on day 4 by eliminating N0 sub( 3) super( -) in f/2 media. There was considerable declining (p