Mechanical unfolding of long human telomeric RNA (TERRA)

Mechanical unfolding of long human telomeric RNA (TERRA)

We report the first single molecule investigation of TERRA molecules. By using optical-tweezers and other biophysical techniques, we have found that long RNA constructions of up to 25 GGGUUA repeats form higher order structures comprised of single parallel G-quadruplex blocks, which unfold at lower...

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Veröffentlicht in:Chemical communications (Cambridge, England) England), 2013-01, Vol.49 (57), p.6397-6399
Hauptverfasser: Garavís, Miguel, Bocanegra, Rebeca, Herrero-Galán, Elías, González, Carlos, Villasante, Alfredo, Arias-Gonzalez, J Ricardo
Format: Artikel
Sprache:eng
Schlagworte:
Chemical communication
G-Quadruplexes
Humans
Repetitive Sequences, Nucleic Acid
RNA - chemistry
RNA - genetics
RNA Folding
Telomere - genetics
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Beschreibung
Zusammenfassung:We report the first single molecule investigation of TERRA molecules. By using optical-tweezers and other biophysical techniques, we have found that long RNA constructions of up to 25 GGGUUA repeats form higher order structures comprised of single parallel G-quadruplex blocks, which unfold at lower forces than their DNA counterparts.
ISSN:1359-7345
1364-548X
DOI:10.1039/c3cc42981d