Improving the understanding of oligonucleotide-nanoparticle conjugates using DNA-binding fluorophores

Improving the understanding of oligonucleotide-nanoparticle conjugates using DNA-binding fluorophores

A novel fluorescent-based method for characterisation of oligonucleotide-nanoparticle conjugates (ONPCs) is reported. We exploit the ability of the double-stranded DNA-binding dye, SYBR Green I, to develop a powerful analytical tool to investigate the melting properties and hybridisation behavior of...

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Veröffentlicht in:Nanoscale 2013-05, Vol.5 (10), p.4166-4170
Hauptverfasser: Guerrini, Luca, Barrett, Lee, Dougan, Jennifer A, Faulds, Karen, Graham, Duncan
Format: Artikel
Sprache:eng
Schlagworte:
DNA - chemistry
Exoribonucleases - chemistry
Fluorescent Dyes - chemistry
Nanoparticles - chemistry
Nucleic Acid Hybridization - methods
Organic Chemicals - chemistry
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Zusammenfassung:A novel fluorescent-based method for characterisation of oligonucleotide-nanoparticle conjugates (ONPCs) is reported. We exploit the ability of the double-stranded DNA-binding dye, SYBR Green I, to develop a powerful analytical tool to investigate the melting properties and hybridisation behavior of ONPCs as well as their corresponding DNA-mediated assemblies.
ISSN:2040-3364
2040-3372
DOI:10.1039/c3nr01197f