Covalent immobilization of the multienzyme enniatin synthetase

Covalent immobilization of the multienzyme enniatin synthetase

The multienzyme enniatin synthetase was covalently immobilized to N-hydroxysuccinimide activated agarose. The stability of the immobilized enzyme at 25 degree C was enhanced compared to the soluble enzyme. Immobilization experiments also indicated that the enniatins are synthesized by a single molec...

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Veröffentlicht in:Biotechnology letters 1985-01, Vol.7 (5), p.297-302
Hauptverfasser: SIEGBAHN, N, MOSBACH, K, GRODZKI, K, ZOCHER, R, MADRY, N, KLEINHAUF, H
Format: Artikel
Sprache:eng
Schlagworte:
Biological and medical sciences
Biotechnology
enniatin synthetase
Enzyme engineering
Fundamental and applied biological sciences. Psychology
Fusarium oxysporum
immobilization
Immobilization of enzymes and other molecules
Immobilization techniques
Methods. Procedures. Technologies
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Zusammenfassung:The multienzyme enniatin synthetase was covalently immobilized to N-hydroxysuccinimide activated agarose. The stability of the immobilized enzyme at 25 degree C was enhanced compared to the soluble enzyme. Immobilization experiments also indicated that the enniatins are synthesized by a single molecule and thus do not require interactions of several enzyme molecules.
ISSN:0141-5492
1573-6776
DOI:10.1007/BF01030274