Macrokinetics of enzyme sorption on porous beads accompanied by complex formation with an immobilized ligand

A mathematical model has been proposed for enzyme sorption on porous beads accompanied by formation of a stable complex with an immobilized ligand. It has been experimentally verified by using the system trypsin (EC 3.2.21.4) - immobilized bovine basic polyvalent trypsin inhibitor on porous silica g...

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Veröffentlicht in:	Enzyme and microbial technology 1985-01, Vol.7 (3), p.111-114
Hauptverfasser:	Bel'nov, V.K., Gromov, A.I., Larionova, N.I., Mityushina, G.V., Safonov, M.S.
Format:	Artikel
Sprache:	eng
Schlagworte:	Analytical biochemistry: general aspects, technics, instrumentation Analytical, structural and metabolic biochemistry basic polyvalent trypsin inhibitor Biological and medical sciences Biotechnology complex formation diffusion Fundamental and applied biological sciences. Psychology immobilized enzymes Mathematical model Methods. Procedures. Technologies trypsin Various methods and equipments
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Beschreibung
Zusammenfassung:	A mathematical model has been proposed for enzyme sorption on porous beads accompanied by formation of a stable complex with an immobilized ligand. It has been experimentally verified by using the system trypsin (EC 3.2.21.4) - immobilized bovine basic polyvalent trypsin inhibitor on porous silica gel. The experimental results for kinetics of the non-specific/specific trypsin sorption on a carrier agree with the model. The value of the coefficient of trypsin diffusion in macroporous silica gel was calculated.
ISSN:	0141-0229 1879-0909
DOI:	10.1016/0141-0229(85)90139-5