Ontogeny of insulin-receptor interaction: Correlation with circulatory insulin levels

Interaction of ( super(125)I)-insulin with intact hepatocytes and its correlation with circulatory insulin level was examined. The hepatocytes from new-born rats bound lowest amount of ( super(125)I)-insulin when circulatory insulin level was high. Hepatocytes from 7 day and 21 day old animals demon...

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Veröffentlicht in:Zeitschrift für Naturforschung C. A journal of biosciences 1983-01, Vol.5 (2), p.125-129
Hauptverfasser: Balapure, A K, Rastogi, A K, Sahib, M K
Format: Artikel
Sprache:eng
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Zusammenfassung:Interaction of ( super(125)I)-insulin with intact hepatocytes and its correlation with circulatory insulin level was examined. The hepatocytes from new-born rats bound lowest amount of ( super(125)I)-insulin when circulatory insulin level was high. Hepatocytes from 7 day and 21 day old animals demonstrated a more or less similar relationship. Cells from 31 day old animals exhibited maximum insulin binding activity against a low serum insulin level. Scatchard analysis of insulin binding shows that the affinity is higher in the hepatocytes from new-born animals than in the hepatocytes of 31 day old animals. Higher binding observed in the latter case may be due to a greater number of binding sites. Hepatocytes from one year old rats bound very little insulin against a high circulatory insulin level. In view of these results, it appears that the down-regulation hypothesis holds true during ontogeny too.
ISSN:0939-5075