Isolation of a calcium-binding protein from an oxygen-evolving photosystem II preparation

Heat treatment of a highly active photosystem II preparation (600 μmol O 2. mg Chl −1.h −1) caused the release of several polypeptides. One of the polypeptides was found to bind to a fluphenazine-Sepharose affinity column in a calcium-dependent manner. The purified polypeptide was able to stimulate...

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Veröffentlicht in:	FEBS letters 1984-11, Vol.177 (1), p.95-98
Hauptverfasser:	Sparrow, Raymond W., England, Reginald R.
Format:	Artikel
Sprache:	eng
Schlagworte:	Biological and medical sciences Calcium calcium-binding protein Calmodulin Cell structures and functions Chloroplast, photosynthetic membrane and photosynthesis Fundamental and applied biological sciences. Psychology Molecular and cellular biology Photosystem II Polypeptide PS, photosystem SDS-PAGE, SDS-polyacrylamide gel electrophoresis
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Zusammenfassung:	Heat treatment of a highly active photosystem II preparation (600 μmol O 2. mg Chl −1.h −1) caused the release of several polypeptides. One of the polypeptides was found to bind to a fluphenazine-Sepharose affinity column in a calcium-dependent manner. The purified polypeptide was able to stimulate calmodulindepleted 3',5'-cyclic nucleotide phosphodiesterase, indicating a possible similarity with calmodulin.
ISSN:	0014-5793 1873-3468
DOI:	10.1016/0014-5793(84)80988-6