The effect of phallotoxins on the structure of F-actin in myosin-free ghost muscle fibres of rabbit

Using polarized UV fluorescent microscopy it has been shown that phallotoxins (phalloidin-sulfone, phalloidin-sulfoxide-B, phalloidin-sulfoxide-A and dithio-phalloidin) cause an increase in tryptophan fluorescence anisotropy of F-actin myofilaments in myosin-free ghost muscle fibres of rabbit. The r...

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Veröffentlicht in:FEBS letters 1984-10, Vol.176 (2), p.441-443
Hauptverfasser: Borovikov, Yu.S., Bukatina, A.E., Son'kir, B.Ja
Format: Artikel
Sprache:eng
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Zusammenfassung:Using polarized UV fluorescent microscopy it has been shown that phallotoxins (phalloidin-sulfone, phalloidin-sulfoxide-B, phalloidin-sulfoxide-A and dithio-phalloidin) cause an increase in tryptophan fluorescence anisotropy of F-actin myofilaments in myosin-free ghost muscle fibres of rabbit. The results obtained are considered to be evidence of conformational changes in F-actin, induced by phallotoxins. These changes are irreversible to a significant extent, which points to a high degree of actin binding to both toxic and nontoxic phallotoxins.
ISSN:0014-5793
1873-3468
DOI:10.1016/0014-5793(84)81214-4