Comparison of a new high-resolution monolithic column with core-shell and fully porous columns for the analysis of retinol and α-tocopherol in human serum and breast milk by ultra-high-performance liquid chromatography
The reduction of analysis time, cost, and improvement of separation efficiency are the main requirements in the development of high‐throughput assay methods in bioanalysis. It can be achieved either by ultra‐high‐performance liquid chromatography (UHPLC) using stationary phases with small particles...
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| Veröffentlicht in: | Journal of separation science 2013-07, Vol.36 (14), p.2223-2230 |
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| container_title | Journal of separation science |
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| creator | Kučerová, Barbora Krčmová, Lenka Solichová, Dagmar Plíšek, Jiří Solich, Petr |
| description | The reduction of analysis time, cost, and improvement of separation efficiency are the main requirements in the development of high‐throughput assay methods in bioanalysis. It can be achieved either by ultra‐high‐performance liquid chromatography (UHPLC) using stationary phases with small particles ( |
| doi_str_mv | 10.1002/jssc.201300242 |
| format | Article |
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It can be achieved either by ultra‐high‐performance liquid chromatography (UHPLC) using stationary phases with small particles (<2 μm) at high back pressures or by using opposite direction—monolithic stationary phases with low back pressures. The application of new types of monolithic stationary phases for UHPLC is a novel idea combining these two different paths. The aim of this work was to test the recently introduced second‐generation of monolithic column Chromolith® HighResolution for UHPLC analysis of liposoluble vitamins in comparison with core‐shell and fully porous sub‐2 μm columns with different particle sizes, column lengths, and shapes. The separation efficiency, peak shape, resolution, time of analysis, consumption of mobile phase, and lifetime of columns were calculated and compared. The main purpose of the study was to find a new, not only economical option of separation of liposoluble vitamins for routine practice.</description><identifier>ISSN: 1615-9306</identifier><identifier>EISSN: 1615-9314</identifier><identifier>DOI: 10.1002/jssc.201300242</identifier><identifier>PMID: 23677738</identifier><language>eng</language><publisher>Weinheim: Blackwell Publishing Ltd</publisher><subject>alpha-Tocopherol - analysis ; alpha-Tocopherol - blood ; Bioanalysis ; Biological and medical sciences ; Chromatography, High Pressure Liquid - instrumentation ; Chromatography, High Pressure Liquid - methods ; Core-shell ; Delivery. Postpartum. Lactation ; Female ; Gynecology. Andrology. Obstetrics ; High-resolution monolith ; Humans ; Investigative techniques, diagnostic techniques (general aspects) ; Liposoluble vitamins ; Maternal, fetal and perinatal monitoring ; Medical sciences ; Milk, Human - chemistry ; Miscellaneous. Technology ; Particle Size ; Pathology. Cytology. 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Sep. Science</addtitle><description>The reduction of analysis time, cost, and improvement of separation efficiency are the main requirements in the development of high‐throughput assay methods in bioanalysis. It can be achieved either by ultra‐high‐performance liquid chromatography (UHPLC) using stationary phases with small particles (<2 μm) at high back pressures or by using opposite direction—monolithic stationary phases with low back pressures. The application of new types of monolithic stationary phases for UHPLC is a novel idea combining these two different paths. The aim of this work was to test the recently introduced second‐generation of monolithic column Chromolith® HighResolution for UHPLC analysis of liposoluble vitamins in comparison with core‐shell and fully porous sub‐2 μm columns with different particle sizes, column lengths, and shapes. The separation efficiency, peak shape, resolution, time of analysis, consumption of mobile phase, and lifetime of columns were calculated and compared. The main purpose of the study was to find a new, not only economical option of separation of liposoluble vitamins for routine practice.</description><subject>alpha-Tocopherol - analysis</subject><subject>alpha-Tocopherol - blood</subject><subject>Bioanalysis</subject><subject>Biological and medical sciences</subject><subject>Chromatography, High Pressure Liquid - instrumentation</subject><subject>Chromatography, High Pressure Liquid - methods</subject><subject>Core-shell</subject><subject>Delivery. Postpartum. Lactation</subject><subject>Female</subject><subject>Gynecology. Andrology. Obstetrics</subject><subject>High-resolution monolith</subject><subject>Humans</subject><subject>Investigative techniques, diagnostic techniques (general aspects)</subject><subject>Liposoluble vitamins</subject><subject>Maternal, fetal and perinatal monitoring</subject><subject>Medical sciences</subject><subject>Milk, Human - chemistry</subject><subject>Miscellaneous. Technology</subject><subject>Particle Size</subject><subject>Pathology. Cytology. Biochemistry. Spectrometry. Miscellaneous investigative techniques</subject><subject>Porosity</subject><subject>Resins, Synthetic - chemistry</subject><subject>Vitamin A - analysis</subject><subject>Vitamin A - blood</subject><issn>1615-9306</issn><issn>1615-9314</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2013</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkc9u1DAQxiMEoqVw5Yh8QeKSxY6TODmiVVv-LOVQEEdrYk8at06c2omWPBYvwgvwMni7y3Lk5PH4931jzZckLxldMUqzt7chqFVGGY-XPHuUnLKSFWnNWf74WNPyJHkWwi2lTFQ1fZqcZLwUQvDqNPm9dv0I3gQ3ENcSIANuSWduutRjcHaeTHzo3eCsmTqjiIq9fiDbeIu1xzR0aC2BQZN2tnYho_NuDgcukNZ5MnUYAbBLMGE3xONkouGD6NfPdHLKjR362DED6eYeBhLQz_0D0HiEMJHe2DvSLGS2k4f04YMj-ugeaYXEmvvZaKI673qY3I2HsVueJ09asAFfHM6z5NvF-df1-3Tz5fLD-t0mVTmtWMorbDTLoRQlq4SmOdSiBq4zgQVArbWqUOkib4Voylo3uqmhQa5LlUELWvGz5M3ed_TufsYwyd4EFdcCA8ZdSJZTymtR5EVEV3tUeReCx1aO3vTgF8mo3AUqd4HKY6BR8OrgPTc96iP-N8EIvD4AEBTY1sd9mPCPE0XJhKgjl--5rbG4_Ges_Hh9veacsyhL9zITJvxxlIG_k6XgopDfry7lxaei2IjPV7LgfwA89dDM</recordid><startdate>201307</startdate><enddate>201307</enddate><creator>Kučerová, Barbora</creator><creator>Krčmová, Lenka</creator><creator>Solichová, Dagmar</creator><creator>Plíšek, Jiří</creator><creator>Solich, Petr</creator><general>Blackwell Publishing Ltd</general><general>Wiley</general><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>201307</creationdate><title>Comparison of a new high-resolution monolithic column with core-shell and fully porous columns for the analysis of retinol and α-tocopherol in human serum and breast milk by ultra-high-performance liquid chromatography</title><author>Kučerová, Barbora ; Krčmová, Lenka ; Solichová, Dagmar ; Plíšek, Jiří ; Solich, Petr</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4081-38ebd14a676187d04a979a3d27e5aa9ddc8ecd54f77b69dbdb9abe3d6c2afadc3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2013</creationdate><topic>alpha-Tocopherol - analysis</topic><topic>alpha-Tocopherol - blood</topic><topic>Bioanalysis</topic><topic>Biological and medical sciences</topic><topic>Chromatography, High Pressure Liquid - instrumentation</topic><topic>Chromatography, High Pressure Liquid - methods</topic><topic>Core-shell</topic><topic>Delivery. Postpartum. Lactation</topic><topic>Female</topic><topic>Gynecology. Andrology. Obstetrics</topic><topic>High-resolution monolith</topic><topic>Humans</topic><topic>Investigative techniques, diagnostic techniques (general aspects)</topic><topic>Liposoluble vitamins</topic><topic>Maternal, fetal and perinatal monitoring</topic><topic>Medical sciences</topic><topic>Milk, Human - chemistry</topic><topic>Miscellaneous. Technology</topic><topic>Particle Size</topic><topic>Pathology. Cytology. Biochemistry. Spectrometry. 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| subjects | alpha-Tocopherol - analysis alpha-Tocopherol - blood Bioanalysis Biological and medical sciences Chromatography, High Pressure Liquid - instrumentation Chromatography, High Pressure Liquid - methods Core-shell Delivery. Postpartum. Lactation Female Gynecology. Andrology. Obstetrics High-resolution monolith Humans Investigative techniques, diagnostic techniques (general aspects) Liposoluble vitamins Maternal, fetal and perinatal monitoring Medical sciences Milk, Human - chemistry Miscellaneous. Technology Particle Size Pathology. Cytology. Biochemistry. Spectrometry. Miscellaneous investigative techniques Porosity Resins, Synthetic - chemistry Vitamin A - analysis Vitamin A - blood |
| title | Comparison of a new high-resolution monolithic column with core-shell and fully porous columns for the analysis of retinol and α-tocopherol in human serum and breast milk by ultra-high-performance liquid chromatography |
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