New approach to study of in vitro toxicity of multiple sclerosis and other sera

The in vitro effects of MS and control sera were quantified by the measurement of radiolabel released from myelinated cultures of rat cerebellum and compared with a visual assessment of myelin damage. Radiolabel release gave a sensitive index of serum effects in vitro which was free of the score ass...

Ausführliche Beschreibung

Gespeichert in:

Bibliographische Detailangaben
Veröffentlicht in:	Journal of the neurological sciences 1984-08, Vol.65 (2), p.157-168
Hauptverfasser:	Bradbury, K., Aparicio, S.R., Sumner, D.W., Bird, C.C.
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Animals, Newborn Biological and medical sciences Cerebellum Culture Techniques Demyelination in vitro Galactosylceramides - metabolism Humans Immune Sera - toxicity Medical sciences Multiple Sclerosis - blood Multiple sclerosis and variants. Guillain barré syndrome and other inflammatory polyneuropathies. Leukoencephalitis Multiple sclerosis serum effects Multiple sclerosis-induced radiolabel release Myelin Sheath - metabolism Myelinating organ cultures Neurology Organ Specificity Rats Tryptophan - metabolism
Online-Zugang:	Volltext
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!

Beschreibung
Zusammenfassung:	The in vitro effects of MS and control sera were quantified by the measurement of radiolabel released from myelinated cultures of rat cerebellum and compared with a visual assessment of myelin damage. Radiolabel release gave a sensitive index of serum effects in vitro which was free of the score assignment decisions that are associated with the visual assessment of myelin damage. Examination of the patterns of radiolabel release elicited by MS and control sera on cultures labelled with either l-[5- 3H]tryptophan or galacto- d-[6- 3H]cerebroside indicates that MS serum effects are not simply a stronger expression of the weak control serum effects.
ISSN:	0022-510X 1878-5883
DOI:	10.1016/0022-510X(84)90080-7