A sensitive, homogeneous fluorescence assay for detection of thymine DNA glycosylase activity based on exonuclease-mediated amplification
A novel homogeneous fluorescence assay strategy for highly sensitive detection of thymine DNA glycosylase (TDG) enzyme activity based on the exonuclease-mediated signal amplification reaction was reported.
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| Veröffentlicht in: | Chemical communications (Cambridge, England) England), 2013-07, Vol.49 (52), p.5874-5876 |
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| container_end_page | 5876 |
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| container_issue | 52 |
| container_start_page | 5874 |
| container_title | Chemical communications (Cambridge, England) |
| container_volume | 49 |
| creator | Chen, Cuihua Zhou, Dianming Tang, Hao Liang, Manfen Jiang, Jianhui |
| description | A novel homogeneous fluorescence assay strategy for highly sensitive detection of thymine DNA glycosylase (TDG) enzyme activity based on the exonuclease-mediated signal amplification reaction was reported. |
| doi_str_mv | 10.1039/c3cc41700j |
| format | Article |
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| fulltext | fulltext |
| identifier | ISSN: 1359-7345 |
| ispartof | Chemical communications (Cambridge, England), 2013-07, Vol.49 (52), p.5874-5876 |
| issn | 1359-7345 1364-548X |
| language | eng |
| recordid | cdi_proquest_miscellaneous_1399917227 |
| source | MEDLINE; Royal Society Of Chemistry Journals 2008-; Alma/SFX Local Collection |
| subjects | Cell Line DNA Probes - metabolism Exonucleases - metabolism HeLa Cells Humans MCF-7 Cells Nucleic Acid Amplification Techniques Spectrometry, Fluorescence Thymine DNA Glycosylase - analysis Thymine DNA Glycosylase - genetics Thymine DNA Glycosylase - metabolism Transition Temperature |
| title | A sensitive, homogeneous fluorescence assay for detection of thymine DNA glycosylase activity based on exonuclease-mediated amplification |
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