A sensitive, homogeneous fluorescence assay for detection of thymine DNA glycosylase activity based on exonuclease-mediated amplification

A novel homogeneous fluorescence assay strategy for highly sensitive detection of thymine DNA glycosylase (TDG) enzyme activity based on the exonuclease-mediated signal amplification reaction was reported.

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Bibliographische Detailangaben
Veröffentlicht in:	Chemical communications (Cambridge, England) England), 2013-07, Vol.49 (52), p.5874-5876
Hauptverfasser:	Chen, Cuihua, Zhou, Dianming, Tang, Hao, Liang, Manfen, Jiang, Jianhui
Format:	Artikel
Sprache:	eng
Schlagworte:	Cell Line DNA Probes - metabolism Exonucleases - metabolism HeLa Cells Humans MCF-7 Cells Nucleic Acid Amplification Techniques Spectrometry, Fluorescence Thymine DNA Glycosylase - analysis Thymine DNA Glycosylase - genetics Thymine DNA Glycosylase - metabolism Transition Temperature
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Beschreibung
Zusammenfassung:	A novel homogeneous fluorescence assay strategy for highly sensitive detection of thymine DNA glycosylase (TDG) enzyme activity based on the exonuclease-mediated signal amplification reaction was reported.
ISSN:	1359-7345 1364-548X
DOI:	10.1039/c3cc41700j