Dissection of antioxidant activity of durum wheat (Triticum durum Desf.) grains as evaluated by the new LOX/RNO method

Antioxidant activity (AA) of durum wheat (Triticum durum Desf.) grains was studied using the innovative LOX/RNO method, able to simultaneously detect different antioxidant mechanisms, and the TEAC assay, one of the most widely used assays. Insoluble-bound and free-soluble phenols, hydrophilic and li...

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Veröffentlicht in:	Journal of cereal science 2012-09, Vol.56 (2), p.214-222
Hauptverfasser:	Laus, Maura Nicoletta, Tozzi, Damiana, Soccio, Mario, Fratianni, Alessandra, Panfili, Gianfranco, Pastore, Donato
Format:	Artikel
Sprache:	eng
Schlagworte:	Antioxidant activity antioxidants Biological and medical sciences Cereal and baking product industries durum wheat Durum wheat grains ferulic acid flour Food industries Fundamental and applied biological sciences. Psychology LOX/RNO method phenols phytochemicals proteins Synergism Triticum aestivum Triticum turgidum subsp. durum
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Zusammenfassung:	Antioxidant activity (AA) of durum wheat (Triticum durum Desf.) grains was studied using the innovative LOX/RNO method, able to simultaneously detect different antioxidant mechanisms, and the TEAC assay, one of the most widely used assays. Insoluble-bound and free-soluble phenols, hydrophilic and lipophilic compounds were extracted from eight different whole flour samples; extracts were analyzed for AA and their content in several antioxidants. The LOX/RNO method measured very high AA values, with the highest ones [850–1500 μmol Trolox eq./g whole flour (dry weight)] for insoluble-bound phenolic extracts, highly correlated to total phenolic (r = 0.761, P
ISSN:	0733-5210 1095-9963
DOI:	10.1016/j.jcs.2012.03.003