Glycoproteins and proteins in an axolemma-enriched fraction and myelin from developing rats: Effect of maternal ethanol consumption

The present study examined proteins and glycoproteins from an axolemma‐enriched fraction from the developing offspring of female rats that were pair‐fed control or 6.6% (50 g/liter) ethanol liquid diets on a chronic basis prior to parturition. In addition, this study examined the synthesis of the major CNS myelin‐associated glycoprotein (MAG) as an index of myelin maturation. The results of the latter study demonstrated normal MAG maturation in ethanol‐treated rats. However, a significant decrease in the proportion of radioactivity associated with MAG was found in the developing offspring of ethanol‐treated rats. The major axolemmal proteins from 32‐day rats included those with molecular weights of 105 K, 81 K, 62 K, 55 K, 52 K, 36 K, and 33 K. Major peaks of radioactivity were associated with fucosylated axolemmal glycoproteins with apparent molecular weights of 150 K, 130 K, 85 K, 76 K, and 64 K. Several development‐related changes in the protein composition of axolemma‐enriched fractions were observed in control animals. Between 22 and 32 days of age control rats exhibited a significant (P