Immunochemical fingerprinting of Clostridium difficile strains isolated from an outbreak of antibiotic-associated colitis and diarrhoea

Department of Bacteriology, University Medical School, Teviot Place, Edinburgh EH8 9 AG, Scotland * Department of Bacteriology, National Bacteriological Laboratory, S-105 21 Stockholm Department of Infectious Diseases, Roslagstulls Hospital, Karolinska Institute, S-104 01 Stockholm, Sweden Received November 17, 1983 Accepted December 2, 1983 SUMMARY. Twenty eight strains of Clostridium difficile , isolated from an outbreak of antibiotic-associated colitis and diarrhoea in an orthopaedic ward and from sporadic cases throughout Sweden, were sent to Edinburgh for immunochemical fingerprinting without information about their origin. EDTA extracts of the organisms were examined by crossed immunoelectrophoresis (CIE), polyacrylamide gel electrophoresis (PAGE) and electroblot transfer. Two patterns were revealed by CIE: group A (18 strains) and group B (10 strains). PAGE and electroblot transfer revealed one major group of 10 strains (group 1), six small groups of two or three strains and six strains which were unlike any other strain. The CIE group B and PAGE-electroblot group 1 were identical. Nine of the 10 strains in this gioup were from patients in the outbreak. These findings indicate that a single strain spread in the orthopaedic ward as a nosocomial infection and that this strain differed from most other strains investigated. The PAGE-electroblot technique should, therefore, greatly aid investigations into the epidemiology of C. difficile infections.