Chemically induced dynamic nuclear polarization studies of guanosine in nucleotides, dinucleotides, and oligonucleotides
The nuclear magnetic resonance (NMR) technique of chemically induced dynamic nuclear polarization (CIDNP) has been used to study the reactions between photoexcited flavins and a wide variety of nucleotides, dinucleotides, and oligonucleotides. The greatest emphasis is placed on the purine nucleoside...
Gespeichert in:
Veröffentlicht in: | Biochemistry (Easton) 1984-01, Vol.23 (9), p.1926-1934 |
---|---|
Hauptverfasser: | , , , , |
Format: | Artikel |
Sprache: | eng |
Schlagworte: | |
Online-Zugang: | Volltext |
Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
Zusammenfassung: | The nuclear magnetic resonance (NMR) technique of chemically induced dynamic nuclear polarization (CIDNP) has been used to study the reactions between photoexcited flavins and a wide variety of nucleotides, dinucleotides, and oligonucleotides. The greatest emphasis is placed on the purine nucleosides adenosine (A), inosine (I), and guanosine (G), particularly guanosine. Strong CIDNP from G is only observed at temperatures above the double-strand melting temperature, suggesting that CIDNP is only detected in single-stranded regions, where the base is accessible to solvent. The use of brominated riboflavin as the the photoreagent in place of riboflavin is shown to selectively invert the sign of A, I, and 1-methylguanosine polarization, providing a convenient method for distinguishing the NMR spectra of these residues in complex oligonucleotides. |
---|---|
ISSN: | 0006-2960 1520-4995 |
DOI: | 10.1021/bi00304a006 |